Rutin ameliorates nitrergic and endothelial dysfunction on vessels and corpora cavernosa of diabetic animals.

Endothelial dysfunction is an early complication of diabetes and it is related to both micro- and macroangiopathies. In addition, >70% of diabetic patients develop autonomic neuropathies. Increased oxidative stress has a major role in the development of both nitrergic and endothelial dysfunction. The aim of this work is to evaluate whether rutin, a potent antioxidant, could ameliorate nitrergic and/or endothelial dysfunction in diabetic animals. Primary and secondary treatment protocols with rutin were investigated on rat aortic rings and the mesenteric arteriolar bed, and on rabbit aortic rings and corpora cavernosa (RbCC) from both euglycemic and alloxan-diabetic animals. Acetylcholine endothelium-dependent and sodium nitroprusside endothelium-independent relaxations were compared in tissues from euglycemic or diabetic animals. Electrical field stimulation (EFS)-induced relaxation was performed only in the RbCC. Endothelial-dependent relaxations were blunted by 40% in vessels and neuronal relaxation was blunted by 50% in RbCC taken from diabetic animals when compared to euglycemic animals. Pre-treatment with rutin restored both neuronal and endothelial dependent relaxations in diabetic animals towards the values achieved in control euglycemic tissues. Rutin was able to ameliorate both endothelial dysfunction and nitrergic neuropathy in animal experimental models. Rutin could be a lead compound in the primary or secondary preventive ancillary treatment of endothelial and nitrergic dysfunction in the course of diabetes.

A high gene flow in populations of Amblyomma ovale ticks found in distinct fragments of Brazilian Atlantic rainforest.

The genetic structure of populations of the tick Amblyomma ovale from five distinct areas of the Brazilian Atlantic rainforest was evaluated via DNA sequencing and associated with the presence of domestic dogs acting as hosts at the edge of forest fragments. Ticks were collected from domestic dogs and from the environment between 2015 and 2017. Four collection areas were located in the surroundings and within the Serra do Mar State Park, São Paulo State (23°37'21"S, 45°24'43"W), where dogs were bimonthly monitored along 2 years using camera traps and GSM trackers. To determine the spatial limits of genetic structure, ticks collected upon dogs living near the Serra do Baturié, Ceará State (4°15'40"S, 38°55'54"W) were included as well. A total of 39 haplotypes of 16S rRNA and Cox 1 mitochondrial genes sequences were observed, with 27 of them coming from areas within the Serra do Mar State Park. No haplotype was shared between the Serra do Mar and the Serra do Baturié indicating isolation of tick populations at the scale of 2000 km. Although three different haplotype lineages of A. ovale occurred within the Serra do Mar State Park, no genetic structure was found across the study sites within this park, suggesting high tick gene flow across a range of 45 km. Monitoring data from domestic dogs and wild carnivores showed that these species share the same habitats at the forest edge, with dogs playing a likely limited role in tick dispersal. Our findings have important implications for understanding the genetic structure of wide spread A. ovale along Brazilian rainforest remnants, which can further be associated to tick-borne infectious agents, such as Rickettsia parkeri, and used for predicting future patterns of tick diversity in the Brazilian Atlantic rainforest.

Riparin B, a Synthetic Compound Analogue of Riparin, Inhibits the Systemic Inflammatory Response and Oxidative Stress in Mice.

The aim of our study was to evaluate the anti-inflammatory, anti-nociceptive, and anti-oxidant action of Riparin B in vivo. We performed experiments in which we induced paw edema by carrageenan and other mediators, carrageenan-induced peritonitis and the level of myeloperoxidase (MPO) activity, pro-inflammatory cytokines (TNF-α and IL-1ß), malondialdehyde (MDA) acid, and glutathione (GSH) from the peritoneal fluid. We also performed behavior tests such as acetic acid-induced writhing, formalin-induced linking, and the hot plate test. Among the doses tested of the Riparin B (1, 3, and 10 mg/kg), the dose of 10 mg/kg showed the strongest effect, and this dose was able to reduce the paw edema induced by carrageenan, dextran, histamine serotonin, bradykinin, 48/80, and PGE2. Similarly, the Riparin B in the same dose reduced cell migration and significantly decreased the nociception induced by formalin and acetic acid and reversed the parameters of the oxidative stress. Thus, we can infer that Riparin B exhibits anti-inflammatory, anti-nociceptive, and anti-oxidant actions in vivo.

[Quality of care in the family healthcare units in the city of Recife: user perception]. / Qualidade do atendimento nas Unidades de Saúde da Família no município de Recife: a percepção do usuários.

This study seeks to assess user perception regarding the quality of care in Family Health Units in Recife. It is a descriptive cross-sectional study adopting a quantitative approach. The survey was conducted in Recife and 939 users were interviewed, being predominantly young adults, female, married, housewives with low levels of education. They have strong links with the units, taking it as a benchmark for their care. The results show care in accordance with scheduled demand and users manifest marked satisfaction with the work of the professionals and less satisfaction with the conditions offered at the units. The younger and more educated the users are, the less satisfied they are with the conditions offered. There is considerable dissatisfaction with delays in attendance, in accessibility and the lack of educational and community activities, the latter two dimensions being related to the core principles of this strategy. The marked satisfaction with clinical care, confidentiality and right to information, which are dimensions related to user-professional relationship are fundamental to the quality of care. This evaluation focused on each aspect of the service provided and makes an important contribution with the critical appraisal reported by users.

Qualidade do atendimento nas Unidades de Saúde da Família no município de Recife: a percepção do usuários / Quality of care in the family healthcare units in the city of Recife: user perception

Este estudo tem por objetivo avaliar a percepção dos usuários com a qualidade do atendimento nas Unidades de Saúde da Família do Recife. Trata-se de um estudo descritivo de corte transversal com abordagem quantitativa. A pesquisa foi realizada no Recife, sendo entrevistados 939 usuários. Os usuários são predominantemente adultos jovens, do sexo feminino, casados, com baixo nível de escolaridade e donas de casa. Apresentam alto vínculo com as unidades, tendo-a como referência para o seu atendimento. Os resultados evidenciam a assistência à demanda programada. Os usuários apresentam maior satisfação com o trabalho dos profissionais e uma menor satisfação com as condições oferecidas pelas unidades. Quanto mais jovem e com mais escolaridade, maior a insatisfação com as condições oferecidas. Apresentaram grande insatisfação com o tempo de espera, com a acessibilidade e com a insuficiência de atividades educativas e comunitárias, sendo as duas últimas dimensões relacionadas aos princípios gerais desta estratégia. A grande satisfação com assistência clínica, confidencialidade e direito a informações, dimensões relacionadas à relação profissional-usuário são determinantes na qualidade da assistência. Esta avaliação enfocando cada momento do atendimento contribuiu na sua apreensão crítica pelos usuários.

This study seeks to assess user perception regarding the quality of care in Family Health Units in Recife. It is a descriptive cross-sectional study adopting a quantitative approach. The survey was conducted in Recife and 939 users were interviewed, being predominantly young adults, female, married, housewives with low levels of education. They have strong links with the units, taking it as a benchmark for their care. The results show care in accordance with scheduled demand and users manifest marked satisfaction with the work of the professionals and less satisfaction with the conditions offered at the units. The younger and more educated the users are, the less satisfied they are with the conditions offered. There is considerable dissatisfaction with delays in attendance, in accessibility and the lack of educational and community activities, the latter two dimensions being related to the core principles of this strategy. The marked satisfaction with clinical care, confidentiality and right to information, which are dimensions related to user-professional relationship are fundamental to the quality of care. This evaluation focused on each aspect of the service provided and makes an important contribution with the critical appraisal reported by users.

Avaliação cefalométrica comparativa dos efeitos dos aparelhos distalizadores Jones Jig, pêndulo e distal Jet - um estudo piloto / Comparative cephalometric evaluation of the effects of distalizing appliances Jones Jig, pendulum and distal jet - a pilot study

O objetivo deste estudo piloto foi avaliar e comparar os efeitos dentários, esqueléticos e nos tecidos moles de três aparelhos distalizadores intrabucais, a saber: Jones Jig, Pêndulo e Distal Jet. Foram selecionados 18 pacientes portadores de má oclusão de classe II dentária, com idade variável de 12,1 a 14,4 anos e divididos, randomicamente, em três grupos de 6 indivíduos.Cada grupo foi tratado com um dos aparelhos acima citados. Cefalogramas pré e pós-tratamento foram obtidos e as diferenças das medidas dentárias, esqueléticas e de tecidos moles foram analisadas pelo teste de Wilcoxon. Houve inclinação distal de coroa dos primeiros molares superiores com significância estatística (p<0,05) para o uso do Jones Jig (8,2º) e do Pêndulo (9,7º). Movimento distal efetivo dos primeiros e segundos molares, 3,3mm e 2,2mm, respectivamente, com significância estatística (p< 0,05) - foi observado apenas para o Distal Jet. Não houve movimento vertical dos molares nem movimento mesial dos pré-molares para nenhum dos três aparelhos. Da mesma forma, alterações esqueléticas e nos tecidos moles não apresentaram significância estatística. Entre os aparelhos testados, o Distal Jet foi o mais eficiente para corrigir a má oclusão de classe II dentária com a distalização efetiva dos primeiros molares superiores através do movimento de translação.

The aim of this pilot study was to evaluate and compare the dental effects, skeletal and soft tissue of three distalizing intraoral appliances, namely: Jones Jig, Pendulum and Distal Jet. We selected 18 patients with Class II malocclusion dental, aged 12.1 to 14.4 anos and divided randomly into three groups of six individuals. Each group was treated with one of the devices mentioned above. Cephalograms before and after treatment were obtained and differences in the measures dental, skeletal and soft tissues were analyzed by Wilcoxon test. There was a crown distal tipping of first molars with statistical significance (p<0.05) for the use of the Jones Jig (8.2 °) and the Pendulum (9.7 °). Effective distal movement of the first and second molars, 3.3 mm and 2.2 mm, respectively, with statistical significance (p<0.05) - it was observed only for the Distal Jet. There was no vertical movement of molars and neither mesial movement of premolars to none of the three devices. Likewise, skeletal and soft tissue changes were not statistically significant.Among the devices tested, the Distal Jet was the most efficient way to correct the Class II dental malocclusion through effective distalization of first molars with translation movement of these teeth.

Qualidade do atendimento nas unidades de saúde da família no Município do Recife: a percepção dos usuários / Quality of care in health units in the family Municipality of Recife: the perception of users

Este estudo aborda uma questão que está na agenda dos gestores da saúde: a qualidade do atendimento prestado e de seus condicionantes. Para o município do Recife, uma avaliação da situação do atendimento na Estratégia de Saúde da Família é de fundamental importância para a consolidação da política em construção. Diante disto, torna-se relevante avaliar como o esforço produzido tem sido percebido pelo principal ator envolvido no processo, o usuário. Trata-se de um estudo descritivo de corte transversal, com abordagem quantitativa. A pesquisa foi realizada no Recife, onde foram entrevistados 939 usuários, distribuídos em 48 ESF de forma proporcional entre os seis distritos sanitários. Os usuários das USF são caracterizados como uma população adulta jovem, do sexo feminino, casada, com baixo nível de escolaridade, dona de casa e que moram no mesmo bairro em que a unidade está localizada. Estes usuários apresentam vínculo com as unidades, tendo esta como referência para o seu atendimento. Foi identificado que o acesso às USF do Recife é decorrente de um bom planejamento do território de cobertura, da organização da demanda e da agilidade nos atendimentos. Percebeu-se que os usuários, apresentam maiores níveis de satisfação com o trabalho dos profissionais, em detrimento as condições oferecidas pelas unidades. Dentre as dimensões da qualidade avaliadas, as ações específicas do PSF, atividades do ACS, visita domiciliar médica e formação de grupos de educação em saúde, apresentaram níveis elevados de insatisfação. Os usuários do DS I apresentaram comportamento diferenciado dos demais distritos, com níveis de satisfação mais elevado. Pode-se concluir com este estudo, que apesar de se verificar nas USF, um movimento dos usuários na busca pela assistência como medida preventiva, de uma solidificação dos vínculos com a unidade e da existência de uma positiva inter-relação com os profissionais...

This study approaches an issue that is on the agenda of health policy- managers: the quality of care provided and their limitations. For the Recife city, an evaluation in the Family Health Strategy treatment situation it is essential for the consolidation of thepolitical construction. Forth this, becomes relevant to assess how the produced effort has been perceived by the main actor involved in the process, the user. This is related to a cross sectional study with a quantitative approach. The survey was conducted in Recife, where 939 users were interviewed, distributed pro rata in 48FHS among the six health districts. Users are characterized as a young adultpopulation, female, married, homemaker, which have a low education level and that live in the same neighborhood where the unit is located. These users are linked to the units, the latter as a reference for your treatment. It was identified that access tothe FHUs in Recife is the result of a good planning about the coverage territory, the organization of demand and agility in emergency. It was noticed that the users have higher levels of satisfaction with the professionals’ work rather than the conditionsoffered by the units. Among the quality dimensions assessed, the specific actions of the FHP, the Auxiliary of Community Health activities, the medical home visits and the formation of groups in health education, had higher levels of dissatisfaction. TheDSI users differ significantly from other districts with higher levels of satisfaction. Based on this study it is possible to conclude that despite being found in the USF, a users movement in the search for assistance as a preventive measure, a solidification of ties with the unity and existence of a positive correlation with theprofessionals, it is noticed the presence of a distortion of the precepts of the family health strategy, bringing to reflection, which really is the role played by family health teams in Recife.

Avaliação da satisfação dos usuários com a qualidade do atendimento nas grandes emergências do Recife, Pernambuco, Brasil / Assessing user satisfaction regarding the quality of health care provided by emergency services in Recife, Pernambuco, Brazil

OBJECTIVES: to assess users' evaluation of the quality of health care provided at emergency services in Recife, in the State of Pernambuco, Brazil. METHODS: a cross-cutting descriptive study covering three major emergency services in the municipality: Hospital da Restauração, Hospital Getúlio Vargas and Hospital Otávio de Freitas. A survey was applied to 420 users (5 percent of the weekly attendance). Data was collected using this survey with closed questions, in an effort to determine the degree of association between the independent variables and the level of user satisfaction. RESULTS: the users surveyed were mostly young adults (46.7 percent), female (59.8 percent), with few schooling years (61.6 percent), working in the home or unemployed (62.3 percent) and living within the Recife Metropolitan Region (83 percent). Dissatisfaction with the conditions provided in hospitals (55.2 percent) was greater among those users with problems stemming from external causes at the Hospital da Restauração, (77.1 percent). The dimensions of quality that received the lowest evaluation were the comfort, 565, and promptness 48.1 percent. CONCLUSIONS: the results show a critical evalua tion from the user's point of view. They also demon strate that there is a clear variation from hospital to hospital. A positive evaluation of staff performance constitutes some hope of improvement; although the other results show that there is still much to be done to improve the quality of care provided by emergency services.

OBJETIVOS: avaliar a satisfação dos usuários em relação à qualidade do atendimento nas grandes emergências do Recife, Pernambuco, Brasil. MÉTODOS: trata-se de um estudo descritivo de corte transversal, realizado nas três maiores emergências do município: Hospital da Restauração, Hospital Getúlio Vargas e Hospital Otávio de Freitas. Foram entrevistados 420 usuários (5 por cento dos atendimentos semanais), através da aplicação de questionários fechados, buscando-se uma relação entre variáveis independentes e o grau de satisfação do usuário. RESULTADOS: o perfil predominante dos usuários foi de uma população adulta jovem (46,7 por cento), do sexo feminino (59,8 por cento), com baixa escolaridade (61,6 por cento), dona de casa ou desempregada (62,3 por cento) e moradora da região metropolitana (83 por cento). A insatisfação com as condições oferecidas esteve presente em 55,2 por cento dos usuários, sendo ainda maior entre aqueles de causa externas da emergência da Restauração (77,1 por cento). As dimensões da qualidade pior avaliadas foram o conforto oferecido, 56 por cento de insatisfação, e a priorização na assistência ao usuário através dos tempos de espera, com 48,1 por cento. CONCLUSÃO: os resultados demonstraram uma avaliação crítica dos usuários. Percebe-se uma nítida variação de resultados entre as três unidades. A avaliação positiva em relação ao trabalho dos profissionais traz um alento sobre as possibilidades, porém os demais resultados evidenciam que muito há por fazer para qualificação do atendimento de emergência.